Anti-Inflammation Evaluation

Creative Biogene Zebrafish Platform offers a cutting-edge and innovative method to discover the relationship between dietary supplements and inflammation. Our expert research team utilizes zebrafish-based models to provide a comprehensive range of scientific evaluations about anti-inflammation efficacy. With years of experience in the field, our team is committed to delivering high-quality research services to facilitate your dietary supplement development and production.

The inflammatory response is a complex biological process that protects the host from infection and injury to maintain homeostasis. However, dysregulated inflammatory responses can be observed in various diseases, leading to chronic inflammation which can significantly impact one's health span. To ward off this threat, more and more people are designing their diets and habits to prevent inflammation. In addition to these efforts, a growing body of evidence suggests that dietary supplements can help keep inflammation in check, thereby preventing or delaying a wide range of age-related diseases. These supplements can be a valuable addition to one's diet and lifestyle, helping them maintain a healthy and balanced inflammatory response.

Zebrafish (Danio rerio) is a popular animal model used in various fields of biomedical research due to its remarkable similarities with humans. Zebrafish is especially valuable for studying inflammation-related research since it carries a highly developed immune system that allows easy observation of inflammatory processes in vivo. The establishment of stable zebrafish inflammation models has made it possible to study inflammation on a deeper level, making zebrafish as a valuable tool for researchers in this field.

Fig. 1 Schematic overview of commonly used zebrafish larval inflammation models

Our Zebrafish-based Scientific Evaluation of Anti-Inflammation Efficacy

Creative Biogene Zebrafish Platform is proud to offer our comprehensive zebrafish inflammatory model as a cutting-edge solution for evaluating the anti-inflammation potential of your dietary supplements. Our team of experts draws on years of experience and expertise to employ three distinct methods - tail transection, CuSO₄ immersion, and LPS injection - to model mechanical, neurologic, and bacterial inflammation respectively. After adding your dietary supplements, we use advanced techniques to evaluate the fluorescence intensity of neutrophils and gene expression, ensuring that your products' anti-inflammation properties are accurately reflected. At Creative Biogene, we are committed to providing the highest level of services and scientific excellence to help you achieve your research goals. Details are as follows.

Example Data

Fig. 2 FA and FB inhibited the migration of neutrophils to the injury site in CuSO4-treated zebrafish. (A) Microphotographs exhibited neutrophils migration in the control, model, FA, and FB groups (neutrophils of 3 dpf transgenic (mpx: EGFP) zebrafish exhibiting green fluorescence). (B) FA and FB dose-dependently reduced the number of neutrophils recruited in the injury site.

Fig. 3 The mRNA expressions of IL-6, IL-1β, and TNF-α in the control, model, FA, and FB groups.

Fig. 4 LCC-09 inhibits LPS-induced accumulation of neutrophils

If you are interested in our services, contact us now to learn more about how we can evaluate the anti-inflammation potential of your dietary supplements.

References

1. Xie, Y.; Meijer, A.H.; et al. Modeling inflammation in zebrafish for the development of anti-inflammatory drugs. Frontiers in cell and developmental biology, 2021, 8: 620984.
2. Gong, L.; Yu, L.; et al. Exploration of anti-inflammatory mechanism of forsythiaside A and forsythiaside B in CuSO4-induced inflammation in zebrafish by metabolomic and proteomic analyses. Journal of Neuroinflammation, 2020, 17: 1-21.
3. Angom, R.S.; Zhu, J.; et al. Wu A T H, et al. LCC-09, a novel salicylanilide derivative, exerts anti-inflammatory effect in vascular endothelial cells. Journal of Inflammation Research, 2021, 14: 4551.

For research use only. Not intended for any clinical use.