Zebrafish Uremic Toxicity Models

Uremia actually means that the human body cannot produce urine through the kidneys, and cannot excrete wastes and excess water produced by metabolism in the body, such as glucose, protein, amino acids, sodium ions, potassium ions, sodium bicarbonate, and acid-base balance. Abnormal, etc. Uremia is a very common severe renal disease in clinical practice, which represents extreme renal failure and has gradually lost the normal metabolic function of the kidneys. Patients with end-stage renal disease (ESRD), including those on dialysis, suffer from a complex array of metabolic disturbances, clinical symptoms, and poor survival. Compared with normal people, ESRD patients have elevated levels of solutes in plasma. However, the relationship between these residual solutes and residual uremia remains poorly understood. Several epidemiological studies have described the relationship between individual uremic solutes and mortality. However, studies on the mechanisms of uremic toxicity are partially limited due to the lack of animal models.

Zebrafish have been widely used to model environmental and pharmacological toxicity for the study of human diseases and potential therapies. Zebrafish exhibit toxicity profiles similar to those of small mammals; toxic concentrations in environmental fish water are consistently similar to toxic serum concentrations in experimental mammals. The mechanism of toxicity of lanthionine, an uncommon amino acid that is increased in uremia patients, has been studied using zebrafish as a model. Studies have demonstrated that morphological changes in zebrafish embryos can be induced early in development, as well as cardiac morphology (increase in atrial size to ventricular ratio) and function (heart rate and arrhythmia propensity) at concentrations comparable to those actually measured in patients. increased significantly). Furthermore, zebrafish produce hundreds of offspring per week, making them a relatively inexpensive and convenient model for simulating uremic toxicity.

Developmental effects of lanthionine and/or cysteine in 72 h post-fertilization zebrafish embryos.

Our Zebrafish Uremic Toxicity Models

Creative Biogene has long-standing experience in establishing preclinical zebrafish models of uremia. We can provide customized zebrafish uremic model construction services to monitor the effects of various compounds on the zebrafish uremic model. Our zebrafish uremic toxicity model will help advance your uremic toxicity mechanism studies and preclinical drug discovery. Contact us to find out what we can do for you!

References

1. Perna AF, et al. Zebrafish, a Novel Model System to Study Uremic Toxins: The Case for the Sulfur Amino Acid Lanthionine. Int J Mol Sci. 2018;19(5):1323.
2. Berman N, et al. A zebrafish model for uremic toxicity: role of the complement pathway. Blood Purif. 2013;35(4):265-269.
3. Stainier DY, et al. Patterning the zebrafish heart tube: Acquisition of anteroposterior polarity. Dev. Biol. 1992;153:91–101.
4. Hill AJ, et al. Zebrafish as a Model Vertebrate for Investigating Chemical Toxicity. Toxicological Sci. 2005;86:6–19.
5. Rubinstein AL, et al. Zebrafish assays for drug toxicity screening. Expert Opinion Drug Metab Toxicol. 2006;2:231–240.

For research use only. Not intended for any clinical use.