Zebrafish Alzheimer's Disease Models

Alzheimer's disease (AD) is a progressive neurodegenerative disease. It is the most common form of dementia in the world, affecting nearly 47 million people worldwide. The two main pathological features of the disease include Aβ plaques and neurofibrillary tangles. Clinically, AD is characterized by progressive memory loss that may include impaired language and motor abilities, depression, delusions, hallucinations, aggressive behavior, and ultimately increased dependence on others before death. Despite extensive research, early diagnosis of AD remains impossible.

The zebrafish (Danio rerio) is a prominent vertebrate model system for comprehensive analysis of the unique functions of genes and their signaling pathways during development and neurodegeneration. Zebrafish possess vertebrate neural architecture, and their genomes have several orthologs of genes that are mutated in familial Alzheimer's disease (FAD) in humans. Furthermore, compared to mammals, the zebrafish brain does have a reasonable level of basic structural protection, as well as similar neuroanatomical and neurochemical pathways that play a role in human disease. Based on the above advantages, the zebrafish (zebrafish) is becoming an increasingly successful model for translational studies of human neurological diseases.

Fig.1 Advantages of using zebrafish as an AD model.

Our Zebrafish Alzheimer's Disease Models

Creative Biogene uses zinc finger nucleases, transcription activator-like effector nucleases (TALENS) and type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas systems for targeted modification of zebrafish Gene sequence genome. We also injected Aβ into the hind ventricle of zebrafish embryos to create a zebrafish AD model in which embryos with marked cognitive deficits and increased Tau phosphorylation at GSK-3β target residues in larvae were observed. The zebrafish has a huge resource of genetic information, and we aim to provide you with a powerful model for research in the field of neuropharmacology and help you uncover the molecular details of AD.

Advantages

• Real-time neuroimaging in zebrafish
• Gene functional analysis at specific time points
• High-throughput gene/drug screening
• Simple and efficient manipulation of multiple genes at physiologically relevant levels

Creative Biogene is committed to providing new insights into the pathogenesis of AD. If you would like to learn more about zebrafish models, please feel free to contact us.

References

1. Newman M, Ebrahimie E, Lardelli M. Using the zebrafish model for Alzheimer's disease research. Front Genet. 2014, 5:189.
2. Saleem S, Kannan RR. Zebrafish: an emerging real-time model system to study Alzheimer's disease and neurospecific drug discovery [published correction appears in Cell Death Discov. 2019 Jul 10;5:116]. Cell Death Discov. 2018, 4:45.
3. Wang X, et al. Advances of Zebrafish in Neurodegenerative Disease: From Models to Drug Discovery. Front Pharmacol. 2021, 12:713963.
4. Pitchai A, Rajaretinam RK, Freeman JL. Zebrafish as an Emerging Model for Bioassay-Guided Natural Product Drug Discovery for Neurological Disorders. Medicines (Basel). 2019, 6(2):61.

For research use only. Not intended for any clinical use.