Zebrafish Genotyping Services
Zebrafish (Danio rerio) is a small vertebrate model widely used by the biomedical research area. Zebrafish has transparent embryos, has rapid development, is inexpensive, can generate a large number of offspring, and has various molecular and imaging tools available. The usefulness of the model was established because of various large forward genetic screens identifying mutants in almost every cell or organ type. Through the increasing development of novel transgenic and gene editing technologies including ZFN, TALENs, and the CRISPR-Cas9 system, the zebrafish is poised to substantially improve the understanding of several pathologic conditions. These technologies have been used to generate targeted knockouts in both somatic and germline cells in zebrafish, efficiently producing genetically modified animals.
In spite of these progresses, traditional genotyping techniques in zebrafish limit the full power of the transgenesis and mutagenesis tools. PCR followed by gel electrophoresis, sometimes combined with restriction enzyme digestion, is widely used to detect genome modification, but is time-consuming and less sensitive to identify small deletions or insertions. With the advances made in zebrafish genome editing, rapid and reliable genotyping has become an indisputable rate-limiting step.
Figure 1. PCR-based HRMA of zebrafish genomic DNA. (Xing L, et al. 2014)
Creative Biogene, a zebrafish research company with experienced scientists and advanced technology, has developed and optimized an automated high-throughput technology that can genotype live zebrafish embryos and larvae. We can provide rapid, high-throughput zebrafish genotyping, point mutation genotyping, and zygosity testing for embryos and adult fish.
Now, we tested and refined a technology to generate cells and usable DNA for genotyping, including PCR analysis, agarose gel electrophoresis, high resolution melt analysis (HRMA), and sequencing. Simply submit fin clips or extracted DNA at room temperature (or on ice) and we will provide validated, guaranteed results within a short time.
Advantages
- Ensure accuracy, reliability, and repeatability, sample after sample
- Built-in redundancy ensures better accuracy
- A quicker, and more sensitive approach to measure and detect mutations
- Spend less on cage real estate
From designing an assay for your study to consulting on results, Creative Biogene is always eager to help. Please email for request forms or for further information.
References
- Lambert C J, et al. An automated system for rapid cellular extraction from live zebrafish embryos and larvae: development and application to genotyping. PloS one, 2018, 13(3).
- Kosuta C, et al. High-throughput DNA extraction and genotyping of 3dpf Zebrafish Larvae by Fin Clipping. JoVE (Journal of Visualized Experiments), 2018 (136): e58024.
- Xing L, et al. Rapid and efficient zebrafish genotyping using PCR with high-resolution melt analysis. JoVE (Journal of Visualized Experiments), 2014 (84): e51138.
For research use only. Not intended for any clinical use.
