Anti-Oxidation Efficacy Evaluation
Creative Biogene Zebrafish Platform is dedicated to providing different ways to test your cosmetic's anti-oxidation capabilities, analyzing the cosmetic in-depth, and offering efficient and integrated services to our clients.
The skin serves as the primary interface between the human body and its surroundings, acting as a barrier against external aggressors. Various environmental factors, such as solar ultraviolet (UV) light, visible light, and environmental pollutants like ozone and particulate matter, increase the generation of reactive oxygen species (ROS) in the skin. Besides exogenous sources that induce reactive species within the skin, the mitochondria also produce ROS as byproducts of oxidative metabolism. Moreover, persistent psychological stress can trigger the occurrence of oxidative stress within the skin by suppressing our antioxidant defense mechanisms. This oxidative stress and the subsequent damage can further intensify skin pigmentation irregularities and accelerate the aging process, leading to alterations in skin tone uniformity, the formation of wrinkles, loss of elasticity, dryness, and coarseness.
Fig. 1 Oxidative stress induced by exogenous and endogenous factors
In the cosmetics industry, there has been a surge in the popularity of compounds that have the potential to mitigate free-radical damage and protect the skin from environmental stressors. Finding antioxidant compounds with high efficacy is a major goal for the cosmetic industry and has attracted a great deal of attention. In recent years, zebrafish has emerged as a valuable model for assessing antioxidant capacities. Zebrafish allows us to study the protective properties of cosmetic active ingredients against external factors.
Our Zebrafish-based Anti-Oxidation Efficacy Evaluation
With years of experience and advanced technologies, Creative Biogene Zebrafish Platform has developed several approaches to evaluate the anti-oxidation capabilities of your cosmetics.
- Reactive oxygen species (ROS) level
Reactive oxygen species (ROS) are highly reactive chemicals formed from diatomic oxygen (O2), water, and hydrogen peroxide, playing an important role in cell signaling. However, during UV exposure, ROS levels increase dramatically, which can cause severe damage to cellular structures.
At Creative Biogene, we divide the zebrafish into several groups for testing, including control group, model group, and test group. The model group and test group are treated with Menadione, while the test group is followed by the addition of the test cosmetic along with Menadione. After using the test cosmetic for a period of time, the zebrafish is subjected to fluorescent staining to observe the production of ROS.
Fig. 2 Antioxidant effects of nutrient combinations. (A) The representative images of yolk sac fluorescence in each group. (B) The fluorescence intensity of the yolk sac in each group (n = 10)
- Superoxide dismutase (SOD) content
Superoxide dismutases (SOD) are a class of enzymes that facilitate the dismutation of superoxide radicals (O2-) into molecular oxygen (O2) and hydrogen peroxide (H2O2), providing cellular defense against ROS. At Creative Biogene, we divide the zebrafish into control group, model group, and test group. The model group and test group are treated with H2O2 while the test group is followed by the addition of the test cosmetic along with H2O2. After using the test cosmetics for a period of time, we will measure the SOD content.
If you are interested in our services, please do not hesitate to contact us at any time and we will provide you with the most professional services for your project.
References
- Chen J, Liu Y, Zhao Z, Qiu J. Oxidative stress in the skin: Impact and related protection. Int J Cosmet Sci, 2021 Oct;43(5):495-509.
- Zhao, B.; et al. Nutrient combinations exhibit universal antianxiety, antioxidant, neuro-protecting, and memory-improving activities. Frontiers in Nutrition, 2023, 9: 996692.
For research use only. Not intended for any clinical use.
