Zebrafish Genome Editing Services
The zebrafish (Danio rerio) has become a popular vertebrate model to study organ formation and function because of its optical clarity and rapid embryonic development. The use of genetically modified zebrafish has also allowed the identification of new putative therapeutic drugs. So far, zebrafish as an animal model has been placed between more reductive in vitro high-throughput screening and beneficial but low-throughput preclinical assays using mammals.
Genome engineering models are essential tools, which are widely used in life science research, including gene function research, cancer research and drug discovery. In particular, in order to faithfully create human disease models, it is advantageous to develop a gene-editing technology that enables predefined and precise sequence modifications in the genome of a model organism. The Clustered, Regularly Interspaced, Short Palindromic Repeat (CRISPR)-associated 9 (Cas9) system has proven to be a simple, efficient and reliable approach for genome editing in zebrafish. CRISPR-Cas9 gene editing has proven to be highly specific, more efficient and easier to customize compared with other methods.
Our Zebrafish Gene-editing Services
Creative Biogene, a zebrafish research company with experienced scientists and advanced technology, can offer multiple gene-editing services including but not limited to point mutations, knockout, short insertions, gene knock-in and conditional knock-in. We use CRISPR-Cas9 (Tol2 available) as our genome editing methods. Our service can significantly reduce your experiment time and can be tailored to different experimental requirements.
- Single and double Knockout
- Conditional Knockout
- Short and large Knockin
- Targeted gene mutation
- Morpholino gene knockdown
- Gene tagging
Application
- Investigation of gene function in the developing zebrafish
- Development of disease models
- Target validation of candidate genes and/or small compounds
- Imaging of organism development and tissue morphology and function
The combined advantages of zebrafish and CRISPR/Cas9 system have become a valuable tool for streamlining the generation of models mimicking human disease, the validation of novel drug targets and the discovery of new therapeutics. For more information or consultation, please feel free to contact us.
References
- Sun Y, et al. Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9. Genome Research, 2020, 30(1): 118-126.
- Li M, et al. Zebrafish genome engineering using the CRISPR–Cas9 system. Trends in Genetics, 2016, 32(12): 815-827.
- Sertori R, et al. Genome editing in zebrafish: a practical overview. Briefings in functional genomics, 2016, 15(4): 322-330.
- Cornet C, et al. Combining zebrafish and CRISPR/Cas9: toward a more efficient drug discovery pipeline. Frontiers in pharmacology, 2018, 9: 703.
For research use only. Not intended for any clinical use.
