Zebrafish Genome Editing Services

The zebrafish (Danio rerio) has become a popular vertebrate model to study organ formation and function because of its optical clarity and rapid embryonic development. The use of genetically modified zebrafish has also allowed the identification of new putative therapeutic drugs. So far, zebrafish as an animal model has been placed between more reductive in vitro high-throughput screening and beneficial but low-throughput preclinical assays using mammals.

Genome engineering models are essential tools, which are widely used in life science research, including gene function research, cancer research and drug discovery. In particular, in order to faithfully create human disease models, it is advantageous to develop a gene-editing technology that enables predefined and precise sequence modifications in the genome of a model organism. The Clustered, Regularly Interspaced, Short Palindromic Repeat (CRISPR)-associated 9 (Cas9) system has proven to be a simple, efficient and reliable approach for genome editing in zebrafish. CRISPR-Cas9 gene editing has proven to be highly specific, more efficient and easier to customize compared with other methods.

Our Zebrafish Gene-editing Services

Creative Biogene, a zebrafish research company with experienced scientists and advanced technology, can offer multiple gene-editing services including but not limited to point mutations, knockout, short insertions, gene knock-in and conditional knock-in. We use CRISPR-Cas9 (Tol2 available) as our genome editing methods. Our service can significantly reduce your experiment time and can be tailored to different experimental requirements.

Single and double Knockout
Conditional Knockout
Short and large Knockin
Targeted gene mutation
Morpholino gene knockdown
Gene tagging

Application

Investigation of gene function in the developing zebrafish
Development of disease models
Target validation of candidate genes and/or small compounds
Imaging of organism development and tissue morphology and function

The combined advantages of zebrafish and CRISPR/Cas9 system have become a valuable tool for streamlining the generation of models mimicking human disease, the validation of novel drug targets and the discovery of new therapeutics. For more information or consultation, please feel free to contact us.

Zebrafish Knockout Services

Creative Biogene provides customized zebrafish knockout model building services, including expert research design, in vitro sgRNA design synthesis, CRISPR vector construction and final zebrafish, allowing you to precisely control the expression of target genes in zebrafish.

Zebrafish Knockin Services

Our expert's team of zebrafish research provides expertise in transgenic manipulation and zebrafish biology for the generation of zebrafish knock-in lines, and subsequent phenotypic analysis for custom drug screening.

Zebrafish Morpholino Gene Knockdown

Zebrafish MO knockdown can be applied to confirm the identity of a proposed target protein, in which case the phenotype of the morphant will mimic the toxic response caused by chemical exposure. Besides, MOs are also excellent tools with which to confirm mechanistic hypotheses generated by exploratory methods.

References

Sun Y, et al. Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9. Genome Research, 2020, 30(1): 118-126.
Li M, et al. Zebrafish genome engineering using the CRISPR–Cas9 system. Trends in Genetics, 2016, 32(12): 815-827.
Sertori R, et al. Genome editing in zebrafish: a practical overview. Briefings in functional genomics, 2016, 15(4): 322-330.
Cornet C, et al. Combining zebrafish and CRISPR/Cas9: toward a more efficient drug discovery pipeline. Frontiers in pharmacology, 2018, 9: 703.