Cytokines and Inflammatory Mediators
Monitor immune and inflammatory responses, supporting disease mechanism research and anti-inflammatory drug evaluation.
- Cell Death and Damage
- Clinical Biochemical Markers
- Cytokines and Inflammatory Mediators
- Endocrine and Hormones
- Growth Factors and Development
- Immune System
- Metabolic Enzymes
- Nervous System
- Other Functional Proteins
- Signal Transduction
- Vascular Function and Angiogenesis
Zebrafish ELISA Kits
OverviewCore FeaturesProduct CategoriesProduct ListQuality ControlFAQ
Zebrafish (Danio rerio) is a widely recognized model organism in life sciences due to its high genomic homology with humans, transparent embryonic development, and rapid life cycle. It plays a vital role in developmental biology, neuroscience, toxicology, and drug screening, providing an ideal platform for unraveling molecular mechanisms and identifying effective drug candidates. The increasing demand for precise quantification of proteins and key biomolecules within limited zebrafish samples poses significant challenges for researchers.
As a global leading supplier in life science reagent development, Creative Biogene specializes in the research and production of zebrafish-specific ELISA kits. Our product portfolio covers popular targets including neurotransmitters, inflammatory factors, cytokines, and enzyme activities, supporting multidisciplinary research needs. Leveraging advanced immunoassay technology and stringent quality control standards, we ensure researchers receive stable, accurate, and reproducible data.
Principle and Advantages of ELISA Technology
Enzyme-Linked Immunosorbent Assay (ELISA) relies on the specific binding between antigen and antibody coupled with enzyme-catalyzed colorimetric reactions to achieve highly sensitive quantification of target molecules. The core steps include:
- Immobilization of capture antibody on microplate wells to specifically bind the target analyte.
- Addition of enzyme-labeled detection antibody to form a stable immune complex.
- Introduction of substrate solution where the enzyme converts a colorless substrate into a colored product.
- Reaction termination and measurement of absorbance with a microplate reader to deduce the target concentration.
Compared to conventional methods, ELISA offers:
- Ultra-high sensitivity with detection limits reaching pg/mL levels, suitable for micro-volume samples.
- Excellent specificity due to high-affinity antibodies minimizing non-specific interference.
- Fast and convenient workflows compatible with high-throughput screening, greatly enhancing experimental efficiency.
- Accurate quantification with robust and reliable data for comparative scientific analysis.
Creative Biogene's zebrafish ELISA kits are optimized for zebrafish-specific sample characteristics, improving detection performance and broadening application versatility.
Core Advantages of Creative Biogene Zebrafish ELISA Kits
High Sensitivity & Reliable Validation
Ultra-low detection limits (0.1 pg/mL–1.0 U/L) with strong reproducibility (R² ≥ 0.99; CVs<10–15%) ensure accurate results from small zebrafish samples.
Efficient & Easy-to-Use
Pre-coated plates and ready-to-use reagents enable quick setup and completion within 90 minutes, streamlining the experimental workflow.
Broad Target Coverage
Covers 100+ zebrafish-relevant biomarkers such as DBH, VEGF-A, and TGF-β, supporting studies in neurotransmission, inflammation, and enzyme activity.
Versatile & Supported
Compatible with diverse sample types (serum, plasma, tissue, supernatants) and backed by expert consultation and custom assay services.
Product Categories Overview
Product List
| Cat.No. | Product Name | ELISA Target Classification | Price |
|---|---|---|---|
| ZEK014 | Zebrafish MT ELISA | Other Functional Proteins | Online Inquiry |
| ZEK014 | Zebrafish MT ELISA | Other Functional Proteins | Online Inquiry |
| ZEK014 | Zebrafish MT ELISA | Other Functional Proteins | Online Inquiry |
| ZEK014 | Zebrafish MT ELISA | Other Functional Proteins | Online Inquiry |
| ZEK014 | Zebrafish MT ELISA | Other Functional Proteins | Online Inquiry |
Rigorous Quality Control Guarantees
Creative Biogene adheres to a strict quality management philosophy to provide high-quality, reliable zebrafish ELISA kits. Our comprehensive quality control system ensures each batch meets the highest standards, enabling precise and efficient research outcomes.
- Comprehensive Quality Inspection: From raw materials procurement, reagent formulation, packaging to final product testing, all processes follow rigorous quality protocols. This includes packaging integrity, accurate labeling, reagent purity and clarity, and volume consistency.
- Advanced Stability Assessment: High-temperature stress tests and multi-batch stability verifications ensure product performance during transport and storage, preserving reagent activity and sensitivity.
- Consistent Intra- and Inter-Batch Precision: Strict control of assay accuracy and reproducibility within and between batches ensures reliable experimental data.
- Validated Standard Curves and Sample Testing: Scientific validation of standard reagents and sample assays guarantees accurate and stable calibration curves.
- Complete Reagent Kit Components and Packaging: Pre-coated microplates are sealed with foil pouches including desiccants for moisture protection. All liquid components are clear without precipitates, preventing nonspecific interference and assuring experimental success.
- Professional Traceability System: Each batch includes detailed quality inspection reports, allowing customers to track production and QC processes, enhancing confidence in product use.
Through this rigorous and scientific quality control, Creative Biogene offers researchers stable, accurate, and efficient ELISA kits as a solid foundation for zebrafish-related life science studies.
Contact Us to Empower Your Research
Creative Biogene is committed to providing professional, efficient, and reliable ELISA solutions for zebrafish research. Whether your focus is basic biology, drug development, or environmental science, we tailor the ideal kit and technical support to meet your unique needs.
FAQ
How do you ensure the accuracy and batch-to-batch consistency of detection results?
We recommend strict adherence to the protocol including reconstitution of standards, sample preparation, and washing steps. Our kits maintain standard curve R² ≥ 0.99 and intra-/inter-assay CVs below 15%. Additionally, we offer experimental guidance and technical support to troubleshoot and maintain assay stability.
Are the kits suitable for the limited sample volume typical of zebrafish? What are the sample collection recommendations?
Our kits are optimized for scarce zebrafish samples and support serum, plasma, tissue homogenates, and cell culture supernatants. Samples should be aliquoted promptly and stored frozen to avoid repeated freeze-thaw cycles. Thaw completely and mix well before testing to ensure sample integrity.
What steps should be taken if abnormal signals or data deviations occur during experiments?
Common causes include sample concentration outside the detection range, inadequate washing, or improper reagent storage. We suggest performing serial dilutions, verifying standard reagent validity, and strictly following the protocol. Creative Biogene's technical team is available for diagnostic support and optimization advice.
Do you offer custom development services and long-term technical support?
Yes. We have extensive experience in custom assay development for specific targets and special sample types. We provide full-cycle technical consultation and after-sales support to ensure smooth research progress and stable supply.
What is the shelf life and storage condition for the kits?
Unopened kits should be stored at 2–8°C with a shelf life of six months. Once opened, reagents should be handled as per instructions to maintain stability and activity.
For research use only. Not intended for any clinical use.
