Zebrafish Chemical-induced Inflammation Models

We noninvasively immerse zebrafish embryos in LPS-containing egg medium or inject into the yolk to establish LPS-induced zebrafish inflammation. We visualize phagocytic cell migration using simple staining in zebrafish or transgenic lines, using quantitative reverse transcription polymerase chain reaction to measure expression levels of key cytokines involved in the inflammatory response, using larval mortality as the primary endpoint. Our LPS-induced zebrafish model of inflammation can be used to study inflammation-mediated diseases, as well as to screen and validate in vivo many compounds that can alter sepsis pathology.

Creative Biogene expose juvenile and adult zebrafish to copper to establish a model of copper-induced inflammation, and we develop an automated high-throughput drug screening assay system based on this model. Depending on your research purpose, we can assess copper pro-oxidative responses and associated necrotic cell death in lateral line hair cells, gill cell apoptosis, monitor neutrophil migration to damaged areas, neutrophil accumulation in neuromas , neurotoxicity and hepatotoxicity and many other indicators.

Creative Biogene inject LTB4 into the ear sac of 3 dpf zebrafish larvae or into the hindbrain at 30 hpf to induce inflammation. In these models we can quantify LTB4-induced neutrophils by counting cells in the pelvic fins, measure systemic LTB4 concentrations in zebrafish by ELISA, determine the expression of genes involved in inflammatory pathways by qPCR, and analyze involvement by microarray experiments Genes of the immune system, genes encoding transcription factors, and genes involved in metabolism.

Creative Biogene has developed a series of zebrafish IBD models, including transgenic zebrafish models of inflammatory responses, chemically induced larval IBD models and chemically induced adult IBD models. Depending on your research goals, we can analyze pro-inflammatory gene expression, assess intestinal structure and motility, monitor morphological changes in enterocytes, and analyze neutrophil counts in the inflamed intestine. We aim to help you analyze the pathogenesis of IBD and screen drug candidates with these zebrafish IBD models.