Protection of Gastrointestinal Mucosa

If you are looking for a reliable solution to evaluate the potential of your dietary supplements in protecting the gastrointestinal mucosa, you can rely on Creative Biogene Zebrafish Platform. Our zebrafish model is highly stable and can help you gain valuable insights into the relationship between your dietary supplements and gastrointestinal mucosa. With our services, you can rest assured that you will receive accurate and actionable information that can help you make informed decisions about your dietary supplements. So why wait? Contact us now to learn more about our services!

In today's fast-paced world, people's lifestyles and dietary patterns have undergone a significant transformation. The rise in the number of gastrointestinal disorders has become a growing concern as a result of these changes. This has led to an increased emphasis on the importance of maintaining good gastrointestinal health.

Zebrafish is a preferred choice in life science research due to several reasons: its small size, high fecundity, early optical transparency, rapid external development, manageable laboratory husbandry, and available genomic resources. The intestines of zebrafish and mammals share a high degree of homology in their development, structural composition, and biological function. The gastrointestinal mucosal barrier system of zebrafish, which boasts a more comprehensive mechanical, chemical, and immune barrier, strongly resembles that of mammals. This similarity makes zebrafish an excellent animal model for gastrointestinal mucosal barrier studies.

Fig. 1 Abdominal anatomy of intestinal tracts between humans A and adult zebrafish (B).

Our Zebrafish-based Scientific Evaluation for Protection of Gastrointestinal Mucosa

TNBS (2,4,6-Trinitrobenzene sulfonic acid) can trigger immune-mediated inflammatory responses in the body, leading to the destruction of the gastrointestinal mucosal barrier and subsequent damage to the gastrointestinal mucosa.

At Creative Biogene, we divide zebrafish into three distinct groups: a vehicle control group, a model group, and a dietary supplement group. Notably, both the model group and dietary supplement group are treated with an identical dose of TNBS. Following the administration of your dietary supplements for a specific duration, we evaluate their gastrointestinal mucosa protective potential through various parameters. These include the evaluation of gastrointestinal lumen area, neutrophil count in the gastrointestinal tract, intestinal goblet cell count, and H&E staining. This comprehensive evaluation ensures accurate and reliable results, providing valuable insights into the efficacy of your dietary supplements in maintaining gastrointestinal health.

Example Data

Fig. 2 (A) Representative images of the mid-intestine of larvae at 10 dpf stained with Alcian Blue. (B) Graph representing the number of goblet cells stained by Alcian Blue at the mid-intestine of 10 dpf larvae.

Fig. 3 ATR reduced H. pylori-induced gastric mucosal damage. (A) H&E-stained histological sections of gastrointestinal tract tissue. (B) Fluorescence microscopy images showing the area of mucosal injury (single-headed arrows indicate the site of analysis of the zebrafish gastrointestinal tract). (C) number of neutrophils.

If you have any questions about our services, please do not hesitate to contact us at any time or submit an inquiry to us directly. We are looking forward to cooperating with you.

References

1. Fénero, C.M.; Amaral, M.A.; et al. Short chain fatty acids (SCFAs) improves TNBS-induced colitis in zebrafish. Current research in immunology, 2021, 2: 142-154.
2. Li, L.; Du, Y.; et al. Atractylone alleviates ethanol-induced gastric ulcer in rat with altered gut microbiota and metabolites. Journal of Inflammation Research, 2022: 4709-4723.

For research use only. Not intended for any clinical use.