Zebrafish Influenza Infection Models

Seasonal influenza virus infections cause epidemics and sporadic epidemics every year. This is a global health problem that causes severe morbidity, mortality and economic burden. Prevention and treatment of influenza disease is difficult due to high rates of viral mutation, emergence of new virus strains, and increased resistance to antiviral drugs. Animal models of influenza infection are critical for our better understanding of the pathogenesis and host response to influenza infection, as well as the screening of antiviral compounds. However, animal models currently used for influenza research, such as mice, ferrets or macaques, are invaluable for studies of transmission, adaptive immunity and vaccine development, but they are not suitable for visualization of host-pathogen interactions or high-throughput drugs filter.

Zebrafish are widely recognized as a valuable model system for infectious disease research and therapeutic drug testing. Early zebrafish were well suited for genetic manipulation, transgenics, and high-throughput drug testing. The zebrafish is completely dependent on its innate immune response during the first 4-6 weeks of development. Since a robust innate immune response enhances the subsequently enhanced adaptive immune response, zebrafish embryos are useful for studying the initial infection of the innate immune system independent of the adaptive immune response. Events provide opportunities. Studies have shown that human influenza A virus (IAV)-infected zebrafish exhibit pathology, including edema and tissue destruction, similar to those observed in human influenza infection, establishing zebrafish as a useful tool for studying human IAV infection. animal model.

Antiviral drug treatment reduces the severity of IAV infection in zebrafish.

Our Zebrafish Influenza Infection Models

Creative Biogene has deep experience in establishing zebrafish influenza infection models whose gross pathology and histopathology can reproduce the clinical symptoms of human influenza infection. In addition, we can provide a zebrafish model of local infection in addition to a model of systemic infection. Using this model of local influenza virus infection, neutrophil recruitment at the site of infection can be tracked, enabling the study of neutrophil biology. Role in influenza virus infection and inflammation. Our zebrafish model can complement existing animal models of human influenza virus infection and is particularly suitable for testing small molecules and immune cell responses.

Advantages

• High optical clarity for easy monitoring of virus infection process
• Support for viral replication
• Reproduction of clinical symptoms of human disease
• Ideal for 4D imaging, genetic screening, and high-throughput drug discovery
• Availability of transgenic lines that label immune cells such as neutrophils
• The ability to track immune cell behavior

Creative Biogene aims to use this model to screen you for potential antiviral drugs, provide valuable insights into host-pathogen interactions across a range of infectious diseases, and advance your understanding of influenza infection and the associated host innate immune response. Please contact us to learn more about our services.

References

1. Gabor KA, et al. Influenza A virus infection in zebrafish recapitulates mammalian infection and sensitivity to anti-influenza drug treatment. Dis Model Mech. 2014,7(11):1227-1237.
2. Sullivan C, et al. Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses. J Vis Exp. 2017, (119):55235.
3. Encinas P, et al. Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach. BMC Genomics. 2010,11:518.

For research use only. Not intended for any clinical use.