Zebrafish High-throughput Screen Services

Zebrafish High-throughput Screen Services

In the last decade, high-throughput chemical screening has become the dominant method for discovering novel compounds with therapeutic properties. Automated screening using in vitro or cultured cell assays have yielded large amounts of candidate drugs for various biological targets. These researches generated many 'hits'; however, recent analysis has suggested that target-based screening has a very low success rate when it comes to identifying potential therapeutic drugs. On the contrary, phenotype-driven screening has a much higher rate of success; thus, the closer one can model the 'natural' environment of cell migration in vivo, the more likely it is to discover novel compounds with potential therapeutic value.

The zebrafish (Danio rerio) has become a crucial model organism in drug discovery as well as in developmental biology. The genetic parallels to humans, combined with the advantage of external fertilization and high fecundity as well as its translucent body, make the zebrafish ideal for scientific research. The development of all major organs progresses rapidly, and hatching takes place 48 to 72 h after fertilization. Moreover, zebrafish can be raised easily and kept alive in standard 96-well plates for several days. Many assays use a large number of zebrafish embryos for high-throughput screening (HTS) by automation technology and computer-aided feature detection.

The core concept of the phenotypic screen is illustrated.

Figure 1. The core concept of the phenotypic screen is illustrated. (Kithcart A, MacRae C A. 2017)

Creative Biogene can create and implement customized, zebrafish high content imaging-based phenotypic assays. For identifying small molecules with biological activity, our zebrafish high-throughput analysis services offer key advantages over cell lines, through providing information on tissue specificity, toxicity, and bioavailability.

Zebrafish-based High Content Screening Applications

  • Screening of zebrafish disease model
  • Gene knockdown quantitation
  • Cardiac function analysis
  • Monitoring inhibition of angiogenesis
  • Measuring ototoxicity
  • Identifying neurotoxicity
  • Targeted image acquisition of a specific area of the well or zebrafish

Advantages

  • Help you generate and advance hits and optimize your leads
  • Maximize throughput for zebrafish-based in vivo screening
  • Optimal flexibility for acquiring high-quality images with a large field of view
  • Deliver optimized preclinical candidates
  • Save time & money

Contact us to learn more about our zebrafish high-throughput screen services. Simply let us know the specific needs and we will propose the best strategy for you.

References

  • Kithcart A, MacRae C A. Using zebrafish for high-throughput screening of novel cardiovascular drugs. JACC: Basic to Translational Science, 2017, 2(1): 1-12.
  • Gallardo V E, et al. Phenotype-driven chemical screening in zebrafish for compounds that inhibit collective cell migration identifies multiple pathways potentially involved in metastatic invasion. Disease models & mechanisms, 2015, 8(6): 565-576.
  • Schutera M, et al. Automated phenotype pattern recognition of zebrafish for high-throughput screening. Bioengineered, 2016, 7(4): 261-265.
  • Spomer W, et al. High-throughput screening of zebrafish embryos using automated heart detection and imaging. Journal of laboratory automation, 2012, 17(6): 435-442.

For research use only. Not intended for any clinical use.

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