Zebrafish Inflammatory Disease Models

Zebrafish Inflammatory Disease Models

The inflammatory response is a complex reflexive process that protects the host against infection and injury to maintain homeostasis. Inflammatory disorders including allergies, autoimmune diseases, asthma and sepsis are major causes of illness and death. It is also becoming apparent that low-grade chronic inflammation underlies many diseases, including type 2 diabetes, cardiovascular disease, cancer, and neurodegeneration, which previously were not considered to possess a strong inflammatory component. Besides, chronic inflammation is also linked with various steps of tumorigenesis and recognized as a risk factor for the occurrence of diverse types of cancers. Treatment of chronic inflammatory diseases like inflammatory bowel diseases and rheumatoid arthritis is still a challenge owing to the lack of safe and effective drugs.

The zebrafish (Danio rerio) has been extensively used in biomedical research as a model to study vertebrate development and hematopoiesis and recently, it has been adopted into multiple fields including immunology. The zebrafish is characterized by high fecundity, ease of observation in the embryonic and larval states, relative ease of genetic manipulation, and low cost of production. Zebrafish has a functional innate immune system at 48 h post-fertilization (hpf) and a mature adaptive system approximately 4-6 wk post-fertilization (wpf), with many of the same immune cells, chemokines and cytokines known in humans. Collectively, zebrafish models possess many advantages of the invertebrate models while also containing a highly developed immune system, which allows for easy visualization of in vivo inflammatory processes and relatively easy high-throughput analyses. Therefore, zebrafish has been an excellent model for the study of inflammatory pathologies.

Creative Biogene has developed a series of zebrafish inflammatory disease model services to help reveal anti-inflammatory activities for candidate drugs. We employed an in vivo phenotypic screen using the zebrafish system to identify previously anti-inflammatory activities for candidate drugs.

  • A fin amputation model of acute inflammation
    Schematic illustrating recruitment and fate of neutrophils during larval tail fin wounding.

    Figure 1. Schematic illustrating recruitment and fate of neutrophils during larval tail fin wounding.

  • Zebrafish inflammatory bowel disease (IBD), including chemically induced adult models and chemically induced larval models
  • Genetic models of obesity and diet induced obesity models

Creative Biogene has the expertise and experience to assist you in assessing the therapeutic potential of your compounds for inflammatory disease. We provide a range of zebrafish model testing systems to promote your drug candidates from discovery to IND. Contact us today to discuss your objectives and how we can reach them.

References

  • Refe Fénero C I M, et al. Inflammatory diseases modelling in zebrafish[J]. World journal of experimental medicine, 2016, 6(1): 9. Rences.
  • Hall C J, et al. Repositioning drugs for inflammatory disease–fishing for new anti-inflammatory agents. Disease models & mechanisms, 2014, 7(9): 1069-1081.
  • Novoa B, Figueras A. Zebrafish: model for the study of inflammation and the innate immune response to infectious diseases. Current topics in innate immunity II. Springer, New York, NY, 2012: 253-275.

For research use only. Not intended for any clinical use.

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