Zebrafish Inflammation Disease Models

The inflammatory response is a complex reflex process that protects the host from infection and injury to maintain homeostasis. Dysregulated inflammatory responses can be observed in various diseases. Abnormal and hyperactivated inflammation plays an important role in the pathogenesis of inflammatory diseases such as asthma, rheumatoid arthritis, and allergic and autoimmune diseases. In addition, chronic inflammation is also associated with various steps of tumorigenesis and is considered to be a risk factor for the development of various cancers. Treating chronic inflammatory diseases such as inflammatory bowel disease and rheumatoid arthritis remains a challenge due to the lack of safe and effective drugs.

The zebrafish (Danio rerio), as a model for studying vertebrate development and hematopoiesis, has recently been used in several fields including immunology. Zebrafish are characterized by high fecundity, easy observation in embryonic and larval states, relatively easy genetic manipulation, and low production costs. Zebrafish have a functional innate immune system at 48 hours post-fertilization (hpf) and a mature adaptive system at approximately 4-6 weeks post-fertilization (wpf) with many of the same immune cells, chemokines and cytokines as humans . Overall, the zebrafish model has many of the advantages of an invertebrate model, while also containing a highly developed immune system that allows easy visualization of in vivo inflammatory processes and relatively easy high-throughput analysis. Therefore, the zebrafish has been an excellent model for studying inflammatory pathology.

Fig 1. Schematic overview of commonly used zebrafish larval inflammation models.

Creative Biogene has developed a range of zebrafish inflammatory disease model services to help reveal the anti-inflammatory activity of drug candidates. We performed an in vivo phenotypic screen using the zebrafish system to identify the previous anti-inflammatory activity of drug candidates.

Zebrafish Wounding-induced Inflammation Models

Fig 2. Zebrafish Wounding-Induced Inflammation Models.

Creative Biogene can provide a variety of traumatic inflammation models such as skin injury, traumatic brain injury, hypoxia/reoxygenation heart injury and traumatic bone injury. These can be used to study fundamental aspects of the inflammatory response, such as the role of specific signaling pathways, migration of leukocytes, and interactions between different immune cells, as well as to screen libraries of natural compounds, approved drugs, and well-characterized pathway inhibitors.

Zebrafish Chemical-induced Inflammation Models

Fig 3. Zebrafish Chemical-Induced Inflammation Models.

Creative Biogene has established a variety of Zebrafish Chemical-Induced Inflammation models, including zebrafish LPS-induced inflammation models, zebrafish copper-induced inflammation models, zebrafish LTB4-induced inflammation models and zebrafish Chemical-Induced Intestinal Inflammation models. We have established high-throughput screening methods based on these models to achieve different types of high-throughput compound screening as well as genetic screening.

Zebrafish Mutation-induced Inflammation Models

Fig 4. Zebrafish Mutation-induced Inflammation Models.

Creative Biogene provides a hai1a mutant zebrafish model (which exhibits a phenotype similar to human psoriasis) and a zebrafish cdipt mutant model (which exhibits characteristics similar to human NAFLD), and we aim to use these model systems for You provide critical support in studying chronic inflammation and displaying high-resolution immune responses in real time.

Creative Biogene has the expertise and experience to assist you in assessing the therapeutic potential of your compounds for inflammatory disease. We provide a range of zebrafish model testing systems to promote your drug candidates from discovery to IND. Contact us today to discuss your objectives and how we can reach them.

References

Morales Fénero CI, et al. Inflammatory diseases modelling in zebrafish. World J Exp Med. 2016, 6(1):9-20.
Hall C J, et al. Repositioning drugs for inflammatory disease–fishing for new anti-inflammatory agents. Disease models & mechanisms. 2014, 7(9): 1069-1081.
Novoa B, Figueras A. Zebrafish: model for the study of inflammation and the innate immune response to infectious diseases. Adv Exp Med Biol. 2012, 946:253-275.
Xie Y, Meijer AH, Schaaf MJM. Modeling Inflammation in Zebrafish for the Development of Anti-inflammatory Drugs. Front Cell Dev Biol. 2021, 8:620984.

For research use only. Not intended for any clinical use.

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