Zebrafish T-cell Acute Lymphoblastic Leukemia Models

T-cell acute lymphoblastic leukemia (T-ALL) is a rare invasive leukemia, which is caused by malignant transformation of hematopoietic progenitor cells. These cells begin to develop into T cells. T-ALL accounts for about 10% to 15% of childhood ALL cases and 25% of adult ALL cases. In the face of severe clinical challenges, scientists have made many efforts to decipher the molecular events behind T-ALL transformation, aiming to identify more specific therapeutic targets and develop more effective and less toxic anti leukemia drugs or drug combinations. The in-depth understanding of T-ALL biology has stimulated the development of T-ALL animal models, and opened up opportunities for in vivo function or preclinical research, with the aim of deciphering the molecular pathology of this disease.

Zebrafish and mammals share a large number of genomes, and zebrafish orthologues have identified 82% of known human pathogenic genes. At present, many zebrafish genes have been proved to reproduce human diseases when affected in zebrafish, including some genes related to hematopoiesis. The first leukemia model of zebrafish is to drive mouse c-Myc oncogene to produce T-ALL through lymphocyte specific rag2 promoter. The success of this model has led to the birth of various other models related to different types of leukemia. Over time, these models have been modified and improved to suit the specific survey of each project, and several similar models are now available.

Fig.1 Advantages of zebrafish model in leukemia research.

Our Zebrafish T-ALL Models

Creative Biogene has many years of experience in zebrafish model research. We can provide transgenic rag2-mMyc zebrafish, conditional tamoxifen induced rag2: Myc-ER fish, transgenic Tg (lck: eGFP) zebrafish and transgenic zebrafish expressing rag2: ICN1-EGFP. These ALL zebrafish models can all show T-ALL phenotype, which is characterized by excessive lymphocyte proliferation, accumulation and infiltration of immature T cell primitive cells in various tissues and organs. Among them, the establishment of conditional tamoxifen induced rag2: Myc-ER fish can improve the analysis and evaluation of the direct causal relationship between Myc oncogene expression and T-ALL. This model follows a similar pattern of disease progression, starting with local T-lymphocyte lymphoma, with slight growth, and then spreading to the circulation and infiltrating other tissues with T-ALL like cells.

It is worth noting that our models can not only help you to analyze the mechanism of T cell transformation in detail, but also can be used to identify new compounds for treating T-ALL. Our service can significantly reduce your experimental time, and can be customized according to different experimental requirements. If you are interested in the zebrafish T-ALL model, please feel free to contact us.

Advantages

• Examine multiple functions of genes in different mutants
• High-throughput gene and drug screening
• Tracking the fate of cancer cells in animals
• High-throughput imaging of adult fish

References

1. Konantz M, et al. Modeling hematopoietic disorders in zebrafish. Dis Model Mech. 2019, 12(9):dmm040360.
2. Baeten JT, de Jong JLO. Genetic Models of Leukemia in Zebrafish. Front Cell Dev Biol. 2018, 6:115.
3. Langenau DM, et al. Myc-induced T cell leukemia in transgenic zebrafish. Science. 2003, 299(5608):887-890.

For research use only. Not intended for any clinical use.