Zebrafish Toxicology Services
Unpredicted human safety events in clinical trials for new drugs are expensive in terms of human health and money. The drug discovery industry tries to minimize those events with diligent preclinical safety testing. Zebrafish toxicology testing is using a much more robust and sensitive model to evaluate vertebrate development pathways and toxicity than small-animal models. The zebrafish has many advantages as a model organism, such as small size, ex utero development of the embryo, and short reproductive cycle. Genotoxicity and developmental toxicity are readily viewable through the transparent embryo during development. Besides, the zebrafish shares a high degree of homology with the human genome.
Cassar S, et al., 2019
High-throughput approaches provided unprecedented opportunities to rapidly identify bioactive compounds. Researchers in the Creative Biogene similarly invented powerful high-throughput screening capabilities to measure complex in vivo phenotypes from chemical exposures at a pace comparable to simple cell-based systems. Besides, the functional roles of many vertebrate gene products in toxicology were discerned in zebrafish using siRNAs, antisense morpholinos, dominant negative gene insertions, and random integration transgenics. The more recent adoption of CRISPR/Cas9 gene editing by Creative Biogene permits the routine interruption of gene expression or replacement of genes, and is emerging as an excellent platform to define the functional role of small nucleotide polymorphisms identified in human Genome Wide Association Studies investigations.
Besides, we can also use zebrafish for studies on eco-environmental monitoring and multitudinous pollutant evaluations, such as toxic heavy metals, endocrine disruptors, and organic pollutants. With the development of transgenic technology, especially the applications of both green fluorescent protein (GFP) and luciferase (LUC) reporters in this field, monitoring efficiency and sensitivity have been dramatically improved.
Our Zebrafish Toxicology Services
SMALL SIZE
Allow the parallel and reproducible testing of several drugs and dosages in simple multiwell plates.
FECUNDITY
Zebrafish reproduce quickly, producing 9,000 offspring in a lifetime.
TRANSLUCENT
Promote direct scoring of organ morphology, and cytological events.
SIMILARITY
Zebrafish are widely used in scientific research for their genetic homology with humans (87% of the genome share).
PHYSIOLOGY
Zebrafish possess recognizable organ systems — livers, hearts, kidneys, pancreases and so on.
References
- Cassar S, et al. Use of zebrafish in drug discovery toxicology. Chemical research in toxicology, 2019, 33(1): 95-118.
- Dai Y J, et al. Zebrafish as a model system to study toxicology. Environmental toxicology and chemistry, 2014, 33(1): 11-17.
For research use only. Not intended for any clinical use.
