Zebrafish B-cell Acute Lymphoblastic Leukemia Models

Acute lymphoblastic leukemia (ALL) is a clonal disease that develops through genetic rearrangements and/or mutations within dominant clones. ALL can be divided into T-cell acute lymphoblastic leukemia (T-ALL) and B-cell acute lymphoblastic leukemia (B-ALL).

Among them, B-ALL is a hematological malignancy derived from immature B cell precursors. It is the most common childhood leukemia and the leading cause of childhood cancer-related deaths. B-ALL can be divided into several subtypes, including pro-B, pre-B, ordinary and mature B-ALL. With increasing incidence, animal models are urgently needed to study the genetics, genomics, drug susceptibility, and other characteristics of ALL to advance therapy development for B-ALL.

Among them, the zebrafish provides a powerful vertebrate model for studying blood diseases. Because the pathway of nodal blood development is highly conserved between zebrafish and mammals, many mutant zebrafish orthologs of human blood disease-related genes have been successfully phenotypically replicated, and with current genomic advances, zebrafish disease models The number is increasing. In addition, the advantages of zebrafish models include their in vitro fertilization and rapid development as well as embryo transparency, facilitating in vivo imaging, and performance for genetic and small molecule screening, properties that allow them to rapidly become models of B-ALL.

Fig.1 Zebrafish B-ALL model responds to dexamethasone and radiation therapy.

Our Zebrafish B-ALL Models

Creative Biogene has many years of experience in zebrafish model research, we can provide TEL-AML1 transgenic zebrafish, Tg(rag2:mMyc) zebrafish and Tg(rag2:hMyc) zebrafish. Among them, transgenic zebrafish expressing TEL-AML1 in lymphoid progenitor cells can mimic human pre-B ALL, identify molecular pathways associated with leukemia development, and even help discover leukemia therapeutic targets. For Tg(rag2:mMyc) zebrafish and Tg(rag2:hMyc) zebrafish, it is worth noting that both models are driven by the transgenic mammalian MYC oncoprotein, and the mMyc and hMYC transgenes used also have a high degree of similarities, but these B-ALLs are actually quite different, occurring in different B cell lineages and with different expression patterns.

Taken together, our models can not only help you deepen your understanding of the leukemia process, but may lead to the identification of new therapeutic targets. Our services can significantly reduce your experimental time and can be customized for different experimental requirements. If you are interested in the zebrafish B-ALL model, please feel free to contact us.

Advantages

• High-throughput gene and drug screening
• Tracking the fate of cancer cells in animals
• Rapidly perform reverse and forward genetic screening
• Visualize cellular processes by in vivo imaging of single cells

References

1. Park G, et al. Zebrafish B cell acute lymphoblastic leukemia: new findings in an old model. Oncotarget. 2020, 11(15):1292-1305.
2. Borga C, et al. Molecularly distinct models of zebrafish Myc-induced B cell leukemia. Leukemia. 2019, 33(2):559-562.
3. Konantz M, et al. Modeling hematopoietic disorders in zebrafish. Dis Model Mech. 2019, 12(9):dmm040360.

For research use only. Not intended for any clinical use.