Zebrafish Copper-induced Inflammation Models

Copper is an essential micronutrient that acts as a catalytic cofactor for various enzymes involved in energy and antioxidant metabolism. As a trace element, the content of copper is strictly controlled, and both its deficiency and excess are harmful to organisms. Under inflammatory conditions, serum copper levels increase and trigger oxidative stress that activates the inflammatory response.

Fig1. Copper-induced inflammation.

A great deal of work has been done in single-cell eukaryotic cells and mammalian cultured cells to understand the underlying mechanisms of copper metabolism. At the same time, significant progress has been made in immunology through the study of genetic pathways in invertebrate models such as worms and flies. In recent years, zebrafish have been used to model inflammation, oxidative stress, apoptosis, and a range of biological and pathological processes due to their developmental, economic, and genetic advantages. In particular, the toxicological and recent pro-inflammatory effects of copper have been studied in larval and adult zebrafish, and breakthrough discoveries have been made. Overall, zebrafish represent a powerful experimental tool for whole-organism approaches, mechanistic studies, disease modeling, and more.

Our Zebrafish Copper-induced Inflammation Models

Creative Biogene exposes juvenile and adult zebrafish to copper to create a model of copper-induced inflammation based on leukocyte aggregation around the neuromammary gland, using double transgenic lines of zebrafish, neutrophils are labeled in red and neuromammary glands are labeled in green . We develop an automated high-throughput drug screening assay system based on zebrafish copper-induced inflammation models. Depending on your research purpose, we can assess copper pro-oxidative responses and associated necrotic cell death in lateral line hair cells, gill cell apoptosis, monitor neutrophil migration to damaged areas, neutrophil accumulation in neuromas , neurotoxicity and hepatotoxicity and many other indicators. We are dedicated to helping you analyze inflammatory responses to different stimuli, drugs, or genetic backgrounds, elucidating copper-related aspects of physiology and disease, and assisting you in making breakthroughs in modulating and regulating immune-related responses.

Our Advantages

• Assessment of transition element distribution in intact zebrafish embryos using X-ray fluorescence microtomography
• Provides automated high-throughput drug screening
• Whole-organism imaging and adaptation to chemical and genetic screening

Creative Biogene not only provides copper-induced inflammation models, but can also design experiments and provide experimental results according to your research goals. If you would like to learn more about zebrafish models, please feel free to contact us.

References

1. Pereira TC, Campos MM, Bogo MR. Copper toxicology, oxidative stress and inflammation using zebrafish as experimental model. J Appl Toxicol. 2016, 36(7):876-885.
2. Chen M, et al. Copper Regulates the Susceptibility of Zebrafish Larvae to Inflammatory Stimuli by Controlling Neutrophil/Macrophage Survival. Front Immunol. 2019, 10:259.
3. Xie Y, Meijer AH, Schaaf MJM. Modeling Inflammation in Zebrafish for the Development of Anti-inflammatory Drugs. Front Cell Dev Biol. 2021, 8:620984.

For research use only. Not intended for any clinical use.