Zebrafish 5-HT ELISA

Online Inquiry

Product Code: ZEK015

Properties
ELISA Target Classification:	Nervous System
Specification:	96T/48T
Sensitivity:	<0.1 ng/mL
Detection Method:	Competitive Method
Specific Principle:	The kit uses a competitive enzyme-linked immunosorbent assay (ELISA). In the pre-coated microplate wells with anti-Zebrafish 5-Hydroxytryptamine (5-HT) antibody (solid-phase antibody), the Zebrafish 5-Hydroxytryptamine (5-HT) calibrator and sample are added, followed by HRP-labeled Zebrafish 5-Hydroxytryptamine (5-HT) antigen (enzyme-labeled antigen). After incubation and thorough washing to remove unbound components, the solid-phase antibody-enzyme-labeled antigen immune complex forms on the microplate surface. Substrate A and B are added, which, under the catalysis of HRP, produce a blue product that turns yellow after being treated with termination solution (2M sulfuric acid). The absorbance (OD value) is measured at 450 nm, and the OD value negatively correlates with the concentration of 5-HT in the sample. A calibration curve is fitted, allowing the calculation of the 5-HT concentration in the sample.
Sample Type:	Serum, plasma, cell supernatant, tissue homogenate
Performance Indicator:	1. Accuracy: The correlation coefficient (R²) of the standard curve ≥ 0.9900 (for most kits) 2. Reproducibility: Intra- and inter-assay coefficient of variation (CV) < 15% 3. Specificity: No cross-reactivity with similar substances
Note:	For accurate information, please refer to the manual or contact customer service.

Description
Storage Conditions:	The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Usage:	For research use only. Not intended for any clinical use.

For research use only. Not intended for any clinical use.