Services
Supported Types
Application
Workflow
Deliverables
Visualize protein expression, cellular distribution, tissue morphology, and pathological changes in zebrafish models with publication-ready imaging and quantitative analysis. We support whole-mount staining, tissue-section staining, histological evaluation, and image-based quantification across embryos, larvae, adult tissues, and cell samples.
This service is ideal for developmental biology, disease modeling, toxicology, immunology, cancer, angiogenesis, injury, and regeneration studies.
Whole-Mount Staining
Tissue Sections
Histology
Image Quantification
Service Options
Histology and Tissue Staining
Evaluate tissue architecture, cellular morphology, and pathological changes with established histological methods:
- H&E Staining
- Masson's Trichrome
- PAS Staining
- Alcian Blue Staining
- Oil Red O Staining
- Sirius Red Staining
- TUNEL Assay
- Customized Staining
Immunofluorescence Staining
Detect and localize specific proteins, cell populations, and biological processes:
- Single-color, dual-color, and multicolor staining
- Whole-mount IF for embryos, larvae, and transparent samples
- Tissue-section IF (paraffin or cryo sections)
- Co-localization analysis
- Fluorescent reporter line imaging
- Nuclear counterstaining (DAPI, etc.)
- Cell counting and fluorescence intensity quantification
Imaging and Quantitative Analysis
Microscopy imaging with objective, reproducible quantification:
- Positive cell counts and percentages
- Fluorescence intensity and integrated density
- Positive area and signal coverage
- Co-localization coefficients
- Tissue injury, lesion, fibrosis, and lipid deposition area
- Blood vessel length, branching, and coverage
- Cell migration distance
- Region-of-interest (ROI) and Z-stack 3D reconstruction
Supported Sample Types
Whole-mount samples
Embryos, larvae, transparent samples, fluorescent reporter fish
Tissue sections
Adult fish tissues, juvenile fish, dissected organs, tumor xenograft samples, injury or regeneration tissues
Cell samples
Primary cells, zebrafish cell lines, cell coverslips, cultured cells
Disease and treatment models
Infection, inflammation, cancer, toxicology, transgenic, gene-edited, drug-treated, and regeneration models
Tissue-section workflows include paraffin or cryo-embedding, sectioning, and slide preparation tailored to sample type.
Applications
Development & Organogenesis
Visualization of embryonic development, organ formation, cell lineage, and gene-editing phenotypes.
Disease Modeling & Toxicology
Assessment of tissue injury, pathological changes, fibrosis, lipid accumulation, and compound-related toxicity.
Immunology & Infection
Localization and quantification of immune cells, inflammatory signals, and host–pathogen interactions.
Cancer & Angiogenesis
Analysis of tumor growth, tumor-associated immune cells, vascular formation, and treatment response.
Injury & Regeneration
Evaluation of cell proliferation, apoptosis, inflammation, tissue repair, and regeneration processes.
Workflow
Deliverables
Depending on service scope, you'll receive:
- Representative stained images (raw + processed/annotated)
- Quantitative results: cell counts, fluorescence intensity, positive area, co-localization data
- Histopathology observations or scoring (where applicable)
- Z-stack images or 3D reconstructions, if included in project scope
- Experimental methods and QC summary
- Final report (PDF) with raw data in spreadsheet format
Sample Requirements
When requesting a project assessment, please provide:
Essential
Research objective · Target markers · Zebrafish strain/stage/genotype · Sample type · Number of groups and biological replicates · Disease model or treatment condition
Helpful
Sample collection and storage details · Preferred antibodies or staining methods · Desired imaging/quantification endpoints · Related qPCR, Western blot, or phenotype data
To request a quotation or feasibility assessment, please send us your target list, zebrafish model information, sample type, group design, sample quantity, and desired analysis endpoints. Our team will recommend an appropriate staining, imaging, and quantification strategy for your study.
For research use only. Not intended for any clinical use.
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