Zebrafish ACA-IgG ELISA

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Product Code: ZEK019

Properties
ELISA Target Classification:	Immune System
Specification:	96T/48T
Sensitivity:	Qualitative (Cut-off value)
Detection Method:	Double Antibody One-Step Sandwich Method
Specific Principle:	The kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). The pre-coated wells with anti-Cardiolipin Antigen antibody are added with samples, HRP-labeled detection antibodies, followed by incubation and thorough washing. The substrate TMB is used for color development, which changes from blue to yellow in the presence of acid due to the catalysis by peroxidase. The absorbance (OD value) is measured at 450 nm and compared to the CUTOFF value to determine the presence of Anti-Cardiolipin Antibody IgG (ACA-IgG) in the sample.
Sample Type:	Serum, plasma, cell supernatant, tissue homogenate
Performance Indicator:	1. Accuracy: The average OD value of the positive control wells ≥ 1.00; the average OD value of the negative control wells ≤ 0.15, indicating that the test results are valid. 2. Specificity: No cross-reactivity with other soluble structural analogs. 3. Reproducibility: Both intra-assay and inter-assay coefficient of variation are less than 15%.
Note:	For accurate information, please refer to the manual or contact customer service.

Description
Storage Conditions:	The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Usage:	For research use only. Not intended for any clinical use.

For research use only. Not intended for any clinical use.