Zebrafish PP2A ELISA
Online InquiryProduct Code: ZEK013
| Properties | |
|---|---|
| ELISA Target Classification: | Signal Transduction |
| Specification: | 96T/48T |
| Sensitivity: | <1.0 U/L |
| Detection Method: | Double Antibody One-Step Sandwich Method |
| Specific Principle: | The kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). The pre-coated wells with anti-Protein Phosphatase 2A (PP2A) antibody are added with samples, standards, and HRP-labeled detection antibodies, followed by incubation and thorough washing. The substrate TMB is used for color development, which changes from blue to yellow in the presence of acid due to the catalysis by peroxidase. The depth of the color correlates positively with the PP2A concentration in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample activity. |
| Sample Type: | Serum, plasma, cell supernatant, tissue homogenate |
| Performance Indicator: | 1. Accuracy: The correlation coefficient (R²) of the standard curve ≥ 0.9900 (for most kits) 2. Reproducibility: Intra- and inter-assay coefficient of variation (CV) < 15% 3. Specificity: No cross-reactivity with similar substances |
| Note: | For accurate information, please refer to the manual or contact customer service. |
| Description | |
|---|---|
| Storage Conditions: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
| Usage: | For research use only. Not intended for any clinical use. |
For research use only. Not intended for any clinical use.
